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Leptospira hardjo antibody EIA 5 x 96 well plate


An ELISA to detect polysaccaride epitope of Leptospira hardjo in serum and milk samples

Article code: EIA B1003-AB01/5



Leptospira hardjo antibody EIA  5 x 96 well plate




Intended use of the testkit
This diagnostic test for leptospirosis is intended to identify antibodies against sugar antigens of Leptospira in serum ans milk samples. In contrast to test systems which make use of umpurified non-specific Leptospira hardjo antigens, this test uses a monoclonal antibody which catches a specific Leptospira hardjo sugar antigen. This monoclonal based ELISA has very high specificity and sensitivity.



Principle of the testkit
An antigen solution antibody mixture is coated to the wells of the micro titer plate after stabilization and drying plates are vacuum sealed. Diluted milk or serum samples are added to the coated wells. After incubation and appropriate washing a monoclonal anti-bovine conjugate is added and the plates are again incubated. After appropriate washing, substrate is added. Within several minutes the color reaction is stopped and the plates are immediately read at 450 nm.



Interpretation of test results
In general high prevalence is more than 15% positive animals. This prevalence can be used for a certain area (f.i. farm, state or country) depending on elimination campaign or other (government) restrictions.

In case of serum samples the following calculation should be used:
       High prevalence:
       A sample is scored Leptospira hardjo negative if the OD  value is below or equal to the average OD value of the mean negative control plus 0,150 OD units.

       * Negative: OD sample < OD mean negative control plus 0,150
       * Positive: OD samples > OD mean negative control plus 0,150

       Low prevalence:
       A sample is scored Leptospira hardjo negative if the OD value is below or equal to the average OD value of the mean negative control plus 0,100 OD units.

       * Negative: OD samples < OD mean negative control plus 0,100
       * Positive: OD samples > OD mean negative control plus 0,200
       * Doubtful: Between mean negative control and 0,200





In case of Milk samples the following calculation should be used:
       High prevalence:
       A sample is scored Leptospira hardjo negative if the OD value is below or equal to the average OD value of the mean negative control plus 0,075 OD units.

       * Negative: OD samples <OD mean negative control 0,075
       * Positive: OD samples > OD mean negative control plus 0,075

       Low prevalence:
       A sample is scored Lewptospira hardjo negative if the OD value is below or equal to the average OD value of the mean negative control plus 0,050 OD units.

       * Negative: OD samples < OD mean negative control plus 0,050
       * Positive: OD samples > OD mean negative 
       * Doubtful: between mean negative control and 0,100



Procedure




€ 1036.48





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